Reporter

Part:BBa_K3304016:Design

Designed by: Platon Megagiannis   Group: iGEM19_Thessaloniki   (2019-10-16)


Amil Cp with BsaI recognition sites that insert PvuII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 16
    Illegal NheI site found at 39
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 740
    Illegal BsaI.rc site found at 4


Design Notes

The part is produced synthetically and can be inserted into bacterial plasmids through the Golden Gate assembly method. Its sequences are produced through our genetic algorithm model to be robust and orthogonal (https://2019.igem.org/Team:Thessaloniki/GeneticA).



Source

For the correct designing of this DNA computing gate, the sequences must be produced and evaluated through our Genetic Algorithm model, while allowing for the inclusion of the recognition sites for both PvuII and Nb.BsrDI enzymes at the appropriate locations.


References